06/20 | General Article | Animal Biotechnology

Diagnosis of Leptospirosis by Polymerase Chain Reaction from Field Specimen in Telangana State

N.Karuna Sree, G.Sunitha, A.V.Krishna Mohan, Ch.Malleswari and V.Lakshma Reddy

Telangana Veterinary Biologicals & Research Institute (TSVBRI), Veterinary & Animal Husbandry Department, Shanthinagar, Hyderabad 500028, Telangana State

Published in the Indian Veterinary Journal July 2020: 97 (7) - pages 12 to 14
(Received: January, 2020, Accepted: March, 2020)


The present study was taken up for rapid diagnosis of Leptospirosis by PCR by processing postmortem specimen collected from Sheep, Goat, Mouse Deer and Cow received from different parts of Telangana State. A total of 48 samples collected from 18 animals (14 Sheep, 1 Goat, 2 Mouse Deer & 1 Cow) were received with a time gap of 2-10 days between collection of specimen at field level and submission to the laboratory were subjected to PCR. 30 samples received with a gap of 2-10 days post collection collected from 9 animals exhibiting clinical signs of Leptospirosis were found positive. 18 samples collected from other 9 animals with the history of grazing over cotton fields were found negative

Key Words: Leptospirosis, PCR, Sheep & Mouse Deer

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