381/18 | General Article | Veterinary Microbiology
Morphological and Molecular Identification of Selected Coleoptera from Sakaka, Aljouf, North-Western Saudi Arabia: Comparative study
A.M.Seufi, Abdulsalam A. Alkhaldi and F.H.GalalDepartment of Biology, College of Science, Jouf University, Sakaka, Aljouf, 2014, Saudi Arabia
Published in the Indian Veterinary Journal April 2019 : 96 (4) - pages 19 to 23(Received: October, 2018, Accepted: November, 2018)
Abstract
Samples of Tenebrionidae and Carabidae were selected from north-western region of KSA. 650 beetles (72.3%) from farms and (27.7%) from houses by trapping/ manual collection. Genus Adesmia (Fam.: Tenebrionidae) showed the most abundance (38.5%).Whilst, both generaAkis (Fam.: Tenebrionidae) and Camedula (Fam.: Carabidae) exhibited the least abundance (1.5%) in this study. DNAs were purified and one or more of six molecular markers (COI, COII, CytB, ND5, 18S and 28S) were amplified over the study. Combining morphological and molecular data, 24 species and 9 genera belonging to 5 tribes, 3 subfamilies, 2 families, 2 superfamilies and 2 suborders. 83.3% of the species belong to the suborder Polyphaga, and 16.7% of the species belong to the suborder Adephaga. In addition, 80% of Polyphaga belong to the subfamily Pimeliinae (Fam.: Tenebrionidae) and only 20% of species belong to the subfamily Tenebrioninae (Fam.: Tenebrionidae).
Key Words: Coleoptera;KSA fauna; molecular identification.