156/17 | General Article | Stem Cell Research and Regenerative Medicine

Clonogenic Assessment on Bone Marrow Derived Mesenchymal Stem Cells

Centre for Stem Cell Research and Regenerative Medicine, Madras Veterinary College, Chennai-600 007, Tamil Nadu.

Abstract

Bone marrow derived Mesenchymal stem cells ( MSCs ) have gained a great deal of attention as a source of autologous, easily isolatable stem cells with profound proliferative capacity, regenerative potential and genetic plasticity. These cells are more suitable with no ethical concerns compared to embryo-derived stem cells for diagnostic and therapeutic applications. Bone marrow derived mesenchymal stem cells have characteristic plastic adherence and are referred as broblastoid colony forming cells as they form characteristic colonies. Their potential ability to form colonies still remains to be an important quality in biomolecule testing operations. The present study describes the comparison of two methods of colony forming potential in sheep bone marrow derived mesenchymal stem cells. It was observed that low initial density of cell seeding has higher CFU potential compared to high initial seeding density in cultural isolation method.

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