342/18 | General Article | Stem Cell Research and Regenerative Medicine
Somatic Cells Acceleration by Platelet Rich Fibrin
Alexander Patera Nugraha, Ida Bagus Narmada, Diah Savitri Ernawati, Aristika Dinaryanti, Helen Susilowati, Eryk Hendrianto, Igo Syaiful Ihsan, Wibi Riawan and Fedik Abdul RantamStem Cell Research and Development Center, Universitas Airlangga, Surabaya - 60132, Indonesia
Published in the Indian Veterinary Journal April 2019 : 96 (4) - pages 30 to 34(Received: August, 2018, Accepted: November, 2018)
Abstract
Gingival Somatic Cells (GSCs) collected from wister rats, were cultured in culture medium, passaged and grown in positive, negative control medium and in experimental medium with different composition for three time duration of 7, 14 and 21 days. Protein Rich Fibrin (PRF) collected from different sources of wister rats were added to the treatment group to assess their effect on the expression of SPARC (Secreted Protein Acidic Rich in Cysteine) which plays a major role in the bone mineralization, cell matricinteraction and collegen bonds, which can be used as markers that correlate with low risk of bone resorption in patients with periodental disease.
Key Words: Gingival Somatic Cells (GSC), Osteogenic differentiation