23/22 | Research Article | Animal Genetics and Breeding

Molecular Epidemiology of Canine Parvovirus (CPV 2) in Dogs in and around Kolkata and Comparison between Field Isolate and Vaccinal Strain

West Bengal University of Animal and Fishery Sciences, 37 & 68, K.B. Sarani, Belgachia, Kolkata West Bengal, India

Abstract

The present study was conducted at Dog Ward of Veterinary Hospital of West Bengal University of Animal and Fishery Sciences, Kolkata campus from December, 2015 to May, 2016 to assess the prevalence of Canine parvovirus infection in Kolkata. Faecal samples were collected from 80 dogs suffering from haemorrhagic gastroenteritis. The samples were subjected to Immunochromatography test, Haemagglutination assay and PCR test. Twenty four dogs were found to be positive for canine parvovirus infection with 30% prevalence. In the present study crossbred dogs (57.14%) were found to be more prone to the disease followed by the Desi breeds (33.33%). In sex wise distribution female dogs were found to be more prone to the disease with 38.46% positivity than male dogs. It was evident that dogs in the age group of 6-12 months were more susceptible to the disease with 41.17% positivity rate. The unvaccinated dogs were having the highest rate of positivity i.e. 41.07% followed by vaccinated i.e. 4.16%.The isolated strains showed 98% homology with CPV type 2a, and CPV type 2c. Moreover, the test samples showed only 86% homology and 14% divergent with the vaccine strain.

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