417/18 | General Article | Veterinary Parasitology
Antigenic Pro le of Excretory / Secretory Antigens of Immature Paramphistomes
R. Vijayashanthi, C. Soundararajan, M. Raman and T.M.A. Senthil KumarDepartment of Veterinary Parasitology, Madras Veterinary College, TANUVAS, Chennai – 600 007, Tamil Nadu
Published in the Indian Veterinary Journal June 2019 : 96 (6) - pages 29 to 31(Received: December, 2018, Accepted: January, 2019)
Abstract
The present study focused on characterization of excretory/secretory antigens of pathogenic immature paramphistomes which could be used for immunodiagnosis of immature paramphistomosis in sheep. The immature ukes were collected from the duodenum of sheep at Perambur slaughter house, Chennai. The ukes were incubated in 10 mM phosphate buffered saline (10 ukes/6ml) at 28.3°C for overnight. The protein concentration varied from 0.236 mg/ml to 0.314 mg/ml using bicinchoninic acid (BCA) method. SDS-PAGE (12%) analysis of the total excretory/secretory antigen of immature ukes revealed 4 immunodominant bands in the range of 10 to 20 kDa which might be used for immunodiagnosis of immature paramphistomosis in sheep.
Key Words: Excretory/Secretory antigens, Immature paramphistomes, SDS-PAGE