417/18 | General Article | Veterinary Parasitology

Antigenic Pro le of Excretory / Secretory Antigens of Immature Paramphistomes

R. Vijayashanthi, C. Soundararajan, M. Raman and T.M.A. Senthil Kumar

Department of Veterinary Parasitology, Madras Veterinary College, TANUVAS, Chennai – 600 007, Tamil Nadu

Published in the Indian Veterinary Journal June 2019 : 96 (6) - pages 29 to 31
(Received: December, 2018, Accepted: January, 2019)


The present study focused on characterization of excretory/secretory antigens of pathogenic immature paramphistomes which could be used for immunodiagnosis of immature paramphistomosis in sheep. The immature ukes were collected from the duodenum of sheep at Perambur slaughter house, Chennai. The ukes were incubated in 10 mM phosphate buffered saline (10  ukes/6ml) at 28.3°C for overnight. The protein concentration varied from 0.236 mg/ml to 0.314 mg/ml using bicinchoninic acid (BCA) method. SDS-PAGE (12%) analysis of the total excretory/secretory antigen of immature ukes revealed 4 immunodominant bands in the range of 10 to 20 kDa which might be used for immunodiagnosis of immature paramphistomosis in sheep.

Key Words: Excretory/Secretory antigens, Immature paramphistomes, SDS-PAGE

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