263/22 | Research Article | Veterinary Public Health and Epidemiology
Efficacy of Vitamin C and E on the Level of Antioxidative Stress and Steroid Hormones in Albino Rats with Environmental Stress
Winda Kusuma Dewi, Faisal Fikri, Agus Purnomo, Shekhar Chhetri, Salipudin Tasil Maslamama, and Muhammad Thohawi Elziyad PurnamaDepartment of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, 60115, Indonesia
Published in the Indian Veterinary Journal January 2023 : 100 (1) - pages 16 to 19(Received: , Accepted: )
Abstract
The aim of this study was to evaluate the efficacy of vitamin C and E in albino rats exposed with environmental stress on the following variables i.e. superoxide dismutase, malondialdehyde, catalase, testosterone, and cortisol level. A total of 20 albino rats were assigned into four treatment groups i.e. (C) normal saline, (T1) vitamin C and E, (T2) induced with stress, (T3) induced with stress and vitamin C and E. All groups were maintained for 4 weeks, respectively. Serum samples were collected from 5 ml of blood, respectively then evaluated using enzymol method for level of antioxidative stress and ELISA method for steroid hormone determination.In result, T3group improved significantly difference compared to T2 group in the variable of malondialdehyde, catalase, testosterone, and cortisol. Meanwhile, there was no significant difference in the superoxide dismutase level between T3 and T2groups. In conclusion, vitamin C and E had an efficacy to ameliorate albino rats condition exposed with environmental stress.
Key Words: environmental stress, oxidative stress, steroid hormone, vitamin C, vitamin E