293/18 | General Article | Veterinary Gynaecology and Obstetrics

Palmitic Acid Supplementation in Tris Extender Can Improve the Freezability of Buffalo Bull Semen

A.K. Singh, H. Kaur, R.S. Cheema, A. Kumar, M. Honparkhe and Prahlad Singh

Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab.

Published in the Indian Veterinary Journal April 2019 : 96 (4) - pages 14 to 16
(Received: August, 2018, Accepted: November, 2018)


This study was designed to evaluate palmitic acid supplementation (0, 2.5, 5.0, 7.5 and 10.0 ng/ml) in extender, on freezability of buffalo bull spermatozoa. Semen ejaculates (n = 24) collected from four buffalo bulls were diluted with Triscitric acid extender containing 0.0 (control), 0.25, 0.5, 0.75 and 1.0 μM palmitic acid and cryopreserved by following established protocol. Sperm progressive motility, viability and plasma membrane integrity were recorded to be higher (P < 0.05) in extender containing 0.5 μM of palmitic acid compared to control and other concentrations. In conclusion, supplementing 0.5 μM palmitic acid in extender, improved the post-thaw quality of buffalo bull semen.

Key Words: Buffalo semen, Tris extender, Cryopreservation

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