78/20 | General Article | Veterinary Gynaecology and Obstetrics
Functional Sperm Parameters of Crossbred Bull Semen Extended Using OptiXcell; a Liposome Based Commercial Extender
S.Sudheer, Deepthi S.S.Rose, S. Sumeeth and V.M.RajeshKerala Livestock Development Board, Mattupatti Semen Station, Idukki District, Kerala 685616
Published in the Indian Veterinary Journal July 2020: 97 (7) - pages 28 to 31(Received: January, 2020, Accepted: April, 2020)
Abstract
A study was undertaken to compare liposome based OptiXcell extender with Tris extender for cryopreservation of crossbred bull semen. Functional sperm parameters were estimated for the semen straws frozen with the two extenders using Computer Assisted Semen Analyzer (CASA) and Flow Cytometer. Sperm motility, live sperms, live sperms with intact Acrosome, sperms with mitochondrial membrane potential and HOS reactive sperms were higher for OptiXcell extender. Altered sperm chromatin structure was lower in OptiXcell compared to Tris. OptiXcell was found to be effective in cryopreservation of crossbred bull spermatozoa and compared to Tris buffer, functional sperm parameters were superior
Key Words: OptiXcell, Sperm cryopreservation, Crossbred bulls