140/19 | General Article | Animal Biotechnology

Non Reversal of Virulence of Live Attenuated Classical Swine Fever Virus During Back Passages in Natural Host

R.Akshaya, K. Kumanan, K.Vijayarani, A. Ramesh and G. Dhinakar Raj

Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai-600007, Tamil Nadu

Published in the Indian Veterinary Journal June 2019 : 96 (6) - pages 12 to 15
(Received: April, 2019, Accepted: May, 2019)


Stability of attenuation of PK-15 cell line adapted Classical swine fever vaccine seed virus was assessed in pigs. Conjunctival and nasal secretions and plasma were screened by Reverse-Transcription Polymerase chain reaction for Classical Swine Fever Virus. Virus was detected in conjunctival and nasal swabs and plasma upto 14 days post inoculation ( DPI ) until III back passage. During 4th passage, the virus excretion was limited only to 3rd and 5th DPI. During 5th passage, virus presence was restricted only to the plasma until 5 DPI. Attenuated virus did not produce any disease during the back passages and found to be safe and stable.

Key Words: Classical Swine Fever virus, PK-15, Live attenuated vaccine

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