277/19 | General Article | Animal Nutrition

In vitro Evaluation of Different Forms of Copper Supplementation on Rumen Microbial Biomass Production

Jha Amitkumar Jitendra, J. Ramesh, R. Karunakaran and R. Anilkumar

Department of Animal Nutrition, Madras Veterinary College, TANUVAS, Chennai-600007, Tamil Nadu, INDIA

Published in the Indian Veterinary Journal August 2020 : 97 (8) - pages 27 to 31
(Received: July, 2020, Accepted: August, 2020)


The present research work was executed with the objective of assessing the impact of different forms of copper (Inorganic: Copper sulphate mega particle, Organic: Copper Methionine and Nano: Copper sulphate) on in vitro microbial biomass production. Copper nano particles with the size of 8.83 nm and zeta potential of -2.43 mV were synthesised by physical method using ball mill. The product yield of nano particle source of copper was 50 g/hour. The current study was carried out to assess the effect of in vitro gas production study on different (Inorganic, organic and Nano) forms of copper on rumen microbial biomass production. Different forms of copper in graded levels (0, 5, 10, 15 and 20 ppm) were added to basal diet and In vitro Apparent Dry matter Digestibility, In vitro True Dry matter Digestibility and Microbial biomass production were assessed by In vitro gas production technique. The results of the present study revealed that supplementation of copper irrespective of form and level resulted in significantly higher (p<0.05) digestibility such as IVADMD, IVTDMD and microbial biomass production compared to non supplemented group. Hence, it was concluded that all the three forms (Inorganic, Organic and Nano) of copper supplementation at 5 ppm level had significantly improved the digestibility of feed.

Key Words: Nano Copper, digestibility, microbial biomass, feed.

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