58/19 | General Article | Donkey

Evaluation of Twelve Heterologous Microsatellite Markers for Parentage Exclusion in Two Donkey Populations of India

R.Behl, S.K. Niranjan, J.Behl, M.S. Tantia, R.K. Vijh and A. Sharma

ICAR - National Bureau of Animal Genetic Resources, P.O. Box 129, GT By-Pass Road, Between ITI and Baldi Chowk, Karnal (Haryana).

Published in the Indian Veterinary Journal June 2020: 97 (6) - pages 9 to 13
(Received: January, 2019, Accepted: April, 2020)


A set of 12 heterologous microsatellite markers was evaluated for parentage exclusion in two Indian donkey populations namely Ladakhi donkeys of Jammu and Kashmir and brown type donkeys of Andhra Pradesh (APB). The genomic DNA from these two donkey populations was amplified using these 12 loci and allelic frequency data so generated was analysed to calculate the probability of paternity exclusion when one of the parents is to be excluded (PE1), both the parents are to be excluded (PE2) and only one parent is known and that is to be excluded (PE3). The cumulative PE1 values taking into consideration all the 12 loci were 0.999750 and 0.999892 in Ladakhi and APB donkeys. The PE2 values with this set were 1-(7.97x10-7) and 1-(1.85x107) in Ladakhi and APB donkeys. These values, being more than the specified minimum exclusion probabilities of 0.9995,indicatedthat this set of 12 microsatellite loci can be safely employed when one of the two parents or both the parents are to be excluded.

Key Words: Indian-donkeys, microsatellite-loci, parentage-exclusion

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