41/22 | Research Article | Veterinary Parasitology
Molecular Identification and Comparative Phylogenetic Analysis of Ehrlichiacanis from Ludhiana, Punjab
Kaushlendra Singh, Harkirat Singh and Nirbhay K. SinghDepartment of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India
Published in the Indian Veterinary Journal December 2022 : 99 (12) - pages 43 to 45(Received: , Accepted: )
Abstract
This study examines the molecular characterisation and comparative phylogenetic analysis of Ehrlichiacanis (Ludhiana isolate) targeting thepartial 16S ribosomal RNA gene. The results of study revealed a very high identity (99–100%) with the gene sequences from Nagpur, Mizoram, and Bareilly isolates from India as well as from those from other countries viz., USA, Japan, Spain, Brazil, Italy, Malaysia, Thailand, Cuba, Austria, Tunisia, Turkey, West Indies, and Israel. However, the lowest identity was observed with E. platys(91.5%) and Anaplasmaphagocytophilum (91.5%).
Key Words: Ehrlichiacanis, PCR, Phylogenetic analysis