78/19 | General Article | Veterinary Gynaecology and Obstetrics

Anti Early Embryonic Protein (EEP) for Pregnancy Test by Microtiter Strip in Dairy Cows

Abdul Samik and Erma Satri

Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia

Published in the Indian Veterinary Journal September 2019 : 96 (09) - pages 37 to 39
(Received: March, 2019, Accepted: May, 2019)


The purpose of this study was to identify and isolate of EEP from cotyledons of pregnant dairy cow for detecting early pregnancy by microtiter strip method in post-inseminated cows at 28 day, including conrmation by serum progesterone and utrasonography. The EEP with a molecular weight of 59.88 kDa could induce the onset of anti-EEP, which can be used for pregnancy test. The EEP microtiter strip test with blood serum progesterone and optimum USG levels was found sensitivity and specicity, as well as had positive predictive values and negative predictive values of 93.33%, 100%, 100% and 83.33%, respectively

Key Words: early embryonic protein (EEP), Pregnancy test, Microtiter strip, Dairy cow

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