219/19 | General Article | Animal Nutrition

Improving the Cell Wall Estimation in Starch Rich Cereal Grains

Sonali Prusty, S.S. Kundu, K.S. Bisitha and U.B. Sontakke and Vijay Kumar Sharma

ICAR-National Dairy Research Institute, Karnal-132001, Haryana.

Published in the Indian Veterinary Journal December 2019 : 96 (12) - pages 38 to 41
(Received: June, 2019, Accepted: September, 2019)


The present study was conducted to work out enzyme concentration and compare the NDF value in cereal grains using heat labile amylase viz. diastase. To determine the requirement of enzyme concentration for complete solubilization of starch, dried ground maize grain samples were treated with graded levels of diastase (activity, 130u/mg) viz. 1, 2, 4, 8, 16 g/L phosphate buffer. The appearance of pale yellow colour at concentration of 8g/L, indicated complete hydrolyzation of starch. With this working enzyme concentration (8g/L) the NDF in starchy grains viz. maize, sorghum, barley, pearl millet, wheat and oats were determined (T1). Other treatments constituted NDF determination using heat stable amylase enzyme (T2) and NDF determination without enzyme pretreatment (T3). The NDF in T2 was signicantly lower than that of T1 and comparable to that of T3. Thus 30ml of the diastase (activity, 130u/mg) solution prepared using 8g diastase/ L phosphate buffer, when incubated overnight was found to be sufcient for starch hydrolyzation in 0.5 g maize sample. Subsequent to the starch hydrolyzation, the conventional detergent method for NDF determination in starchrich grains consequently yielded precise results.

Key Words: Amylase, Grain, Neutral detergent  bre, Themolabile, Thermostable

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