Research Article | Livestock Production Management

Enhancements in the Diagnosis of Mastitis in Cows, held in an Intensive Farming System

Fedotov SergeyVasilievich, Regan Reddy Gade*, SidnevNikita Iurevich, Fouad sakr, and Zherebtsov Ilya Sergeevich

Department of Disease Diagnosis, Therapy, Obstetrics and Reproduction of Animals FSBEI HE Moscow State Academy of Veterinary Medicine and Biotechnology-MVA named after K.I. Skryabin. Akalemik Scriabin

Published in the Indian Veterinary Journal July 2022 : 99 (7) - pages 39 to 46
(Received: , Accepted: )


The specific composition of the microflora of the udder was studied in cows with suspicion ofmastitis with the help of PCR diagnostics, kept in two complexes at JSC«Lednevo» with extensive and intensive method. In cows kept under the extensive method, with mastitis, microorganisms were isolated more often in associations than in monoculture. In most samples, E. coli (9.01%) was isolated in monoculture, as well as in association in 36.36% of cases. Microorganisms of the genus Staphylococcus, Streptococcus uberis, Mycoplasma mycoides, and Chlamydiaceae were the other most common in associations. When using modern milking systems with untethered (intensive method), coccal microflora prevailed in the PCR examination of milk from cows with mastitis. Based on the analysis of the results regarding the analysis of milk obtained from cows, the dependence of changes in productive qualities, physicochemical and technological properties, qualitative composition, as well as microbiological, sanitary and hygienic indicators of milk with respect to mammary glands pathology was established, which can be used as an additional test for diagnosis of subclinical mastitis. With an increase in the number of mastitis in cows in large livestock complexes with an intensive housing system, it is advisable to use modern PCR diagnostics. Real-time PCR has significant advantages over traditional methods, as it has higher sensitivity and accuracy.

Key Words: PCR, diagnostics, milk, staphylococcus, streptococcus uberis, Mycoplasma mycoides, Chlamydia cease,

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