139/19 | General Article | Poultry Science

Efciency of W Chromosome- Based Gender Determination in Japanese Quails

Tamadhur H. Hussein, Mohammed Baqur S. Al-Shuhaib and Tahreer M. Al-Thuwaini

Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil 51001, Iraq.

Published in the Indian Veterinary Journal November 2019 : 96 (11) - pages 36 to 39
(Received: April, 2019, Accepted: July, 2019)


The current research was designed to differentiate between males and females using the recently published referring sequences of the female W chromosome. Specic PCR primers were designed to amplify 233 bp and 170 bp of AKAP8 gene, and WIZ gene, respectively. All the results revealed no differences between both sexes since both W chromosome-specic PCR primers were found to amplify other somatic positions within the included males. However, extremely high specicity was observed in quails’ identity detection which suggests that both designed AKAP8 and WIZ amplicons represent highly attractive markers for this species identication

Key Words: AKAP8 gene, quails sexing,WIZ gene, W chromosome

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