226/22 | Research Article | Veterinary Microbiology

Phenotypic and Genotypic Diversity of Indigenous Limosilactobacillus fermentum isolated from “Thairu”-Traditional Fermented Milk Product of Kerala

Lakshmi Shaji, ArchanaChandran, Aswini K.V, and Sumi Mary George

Department of Dairy Microbiology,College of Dairy Sciences and Technology,Kerala Veterinary and Animal Sciences University,Pookode, 673576, Kerala, India

Published in the Indian Veterinary Journal November 2022 : 99 (11) - pages 40 to 44
(Received: , Accepted: )


Limosilactobacillus fermentum sp is a predominant lactic acid bacterium in the microflora of curd/dahi. The identification and classification of it plays a significant role in understanding the diversity of microbes in traditional fermented milks. There is little data available on the diversity within this species in our state. Hence an attempt was made to study the phenotypic and genotypic diversity of this species. The phenotypic characterizations such as morphology, Gram reaction, colony characteristics, fermentation pattern in milk, flavour production and antibiotic susceptibility were done. For genotypic studies RAPD- PCR was used as a tool with the random primer KAY-3.The results of the study clearly revealed the similarity among strains ADMH 12, ADMH 14 and ADMH 20. ADMH 18 and ADMH 25 showed similar characteristics. The study throws light on the fact that environment plays a significant role in modification of genetic makeup of organisms so as to adapt to different geographical and climatic conditions.

Key Words: Lactic acid bacteria, curd, antibiotic, RAPD-PCR

Main Article

Access to the IVJ Digital Archives is restricted to our paid subscribers. Please consider becoming a subscriber to continue reading this article.