02/20 | General Article | Veterinary Microbiology

Antimicrobial Activity of Moringa oleifera against Avian Pathogen and Determination of its Bioactive Compounds using GC-MS

Department of Microbiology, K.S.Rangasamy College of Arts and Science (Periyar University), Tiruchengode – 637 215, Tamilnadu

Abstract

The isolation and identication of avian pathogens was undertaken by utilizing 780 samples from 460 layers suffering from respiratory diseases and enteritis. The antimicrobial activity of extracts of Moringa oleifera leaves was evaluated against avian pathogen viz. Escherichia sp., Klebsiella sp., Salmonella sp., Pasteurella sp., Staphylococcus sp. and Aspergillus sp. isolated from layer chicken and characterization of its chemical constituents was determined by qualitative phytochemical analysis and GC-MS. Antimicrobial activity of methanol, ethanol, petroleum ether and aqueous extracts of leaves of this plant was determined by disc diffusion method. The methanol extracts of M. oleifera exhibited signi cant antimicrobial activity against isolated avian microorganisms. The leaves of M. oleifera possessed 22 bioactive compounds in total and 6 compounds have showed antimicrobial activity and could be used effectively to develop a novel herbal antimicrobial drug against avian pathogens.

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