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Encoding Gene for Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Nasal Swab of Dogs

Reina Puspita Rahmaniar, Maya Nurwartanti Yunita, Mustofa Helmi Effendi, and Sheila Marty Yanestria

Department of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia

Published in the Indian Veterinary Journal February 2020: 97 (2) - pages 37 to 40
(Received: September, 2019, Accepted: October, 2019)


The purpose of this study was to isolate and identify encoding gene for methicillin-resistant Staphylococcus aureus (MRSA) from nasal swab of dogs in Surabaya, Indonesia. Nasal swab of dogs of 85 samples obtained from ve areas in Surabaya. Bacterial identication was based on the growth in Mannitol Salt Agar, Gram staining, catalase, coagulase and VP tests. 43 (50.59%) out of 85 samples were for positive Staphylococcus aureus isolation. MRSA conrmation by Oxacillin Resistant Screen Agar Base (ORSAB) were 25(29.41%). The molecular identication on mecA gene by PCR showed that 5(5.88%) isolates were positive contain mecA gene. It was concluded that the dogs as companion animals can be a potential reservoir for MRSA strains to threat public health

Key Words: Staphylococcus aureus, MRSA, Dogs, mecA gene

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