250/19 | General Article | Veterinary Pathology

Characterization and Production of Polyclonal Antibody Anti Excretory Secretory Protein of Blastocystis sp

Briantono Willy Rendragraha, Lucia Tri Suwanti, Rahadju Ernawati, Mufasirin Mufasirin, Setiawan Koesdarto, Wiwiek Tyasningsih, Soelih Estoe Pangestie, Heni Puspitasari, and Septian Hakim Susantoputro

Faculty of Veterinary Medicine, Airlangga University, Kampus C Unair, Mulyorejo, Surabaya 60115, East Java, Indonesia

Published in the Indian Veterinary Journal December 2019 : 96 (12) - pages 30 to 32
(Received: July, 2019, Accepted: August, 2019)


This study aims to produce and characterize polyclonal antibody anti excretory secretory (ES) protein of Blastocystis sp. ES protein pro le was analyzed using SDS-PAGE and used to immunize 2 rabbits. Rabbit’s serum were analyzed using indirect ELISA and Western Blot. The result showed that molecular weight of ES protein of Blastocystis sp was 40 and 50 kDa and the protein was immunogenic. Both ES protein and antibody anti ES of Blastocystis sp can be promoted as diagnostic kit

Key Words: Blastocystis sp, Excretory Secretory Protein, Polyclonal Antibody

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