250/19 | General Article | Veterinary Pathology
Characterization and Production of Polyclonal Antibody Anti Excretory Secretory Protein of Blastocystis sp
Briantono Willy Rendragraha, Lucia Tri Suwanti, Rahadju Ernawati, Mufasirin Mufasirin, Setiawan Koesdarto, Wiwiek Tyasningsih, Soelih Estoe Pangestie, Heni Puspitasari, and Septian Hakim SusantoputroFaculty of Veterinary Medicine, Airlangga University, Kampus C Unair, Mulyorejo, Surabaya 60115, East Java, Indonesia
Published in the Indian Veterinary Journal December 2019 : 96 (12) - pages 30 to 32(Received: July, 2019, Accepted: August, 2019)
Abstract
This study aims to produce and characterize polyclonal antibody anti excretory secretory (ES) protein of Blastocystis sp. ES protein pro le was analyzed using SDS-PAGE and used to immunize 2 rabbits. Rabbit’s serum were analyzed using indirect ELISA and Western Blot. The result showed that molecular weight of ES protein of Blastocystis sp was 40 and 50 kDa and the protein was immunogenic. Both ES protein and antibody anti ES of Blastocystis sp can be promoted as diagnostic kit
Key Words: Blastocystis sp, Excretory Secretory Protein, Polyclonal Antibody