356/19 | General Article | Animal Biotechnology

Restriction Fragment Length Polymorphism on Growth Hormone Gene of Madrasin Crossbreed Cattle

GheaAquaticaPuteri, Budi Utomo and RoesnoDarsono

Faculty of Veterinary Medicine, UniversitasAirlangga, Surabaya-60115, Indonesia

Published in the Indian Veterinary Journal March 2020: 97 (3) - pages 23 to 26
(Received: October, 2019, Accepted: October, 2019)


This research aims to identify GH gene polymorphism in cattle as a result of cross-breeding between Madura and Limousine cattle (as Madrasin cattle) using Restriction Fragment Length Polymorphism (RFLP). Blood samples of 14Madrasin cattle were taken for DNA isolation, DNA ampli cation with GH primers by using PCR-RFLP method. Restriction enzyme was AluI. The results suggested L allele frequency reached 0.96 while V allele reached 0.075. Therefore, LL genotype was acquired, amounting to 0.928, with LV amounting to 0.017. It can be concluded that there is a polymorphism in GH genes of Madrasin cattle (L and V alleles).

Key Words: Growth hormone gene, Madrasin cattle, PCR-RFLP, polymorphism

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