356/19 | General Article | Animal Biotechnology
Restriction Fragment Length Polymorphism on Growth Hormone Gene of Madrasin Crossbreed Cattle
GheaAquaticaPuteri, Budi Utomo and RoesnoDarsonoFaculty of Veterinary Medicine, UniversitasAirlangga, Surabaya-60115, Indonesia
Published in the Indian Veterinary Journal March 2020: 97 (3) - pages 23 to 26(Received: October, 2019, Accepted: October, 2019)
Abstract
This research aims to identify GH gene polymorphism in cattle as a result of cross-breeding between Madura and Limousine cattle (as Madrasin cattle) using Restriction Fragment Length Polymorphism (RFLP). Blood samples of 14Madrasin cattle were taken for DNA isolation, DNA ampli cation with GH primers by using PCR-RFLP method. Restriction enzyme was AluI. The results suggested L allele frequency reached 0.96 while V allele reached 0.075. Therefore, LL genotype was acquired, amounting to 0.928, with LV amounting to 0.017. It can be concluded that there is a polymorphism in GH genes of Madrasin cattle (L and V alleles).
Key Words: Growth hormone gene, Madrasin cattle, PCR-RFLP, polymorphism