59/21 | General Article | Veterinary Biochemistry
Isolation and Purification of Bovine Lactoferrin from Milk of Poda Thurpu Breed of Telangana
Mohammed Shaz Murtuza, Ashok Kumar Devarasetti1, Akkaladevi Jayasri, B.D.P Kalakumar, Ugandar Reddy Gajjalaiahvari and Lavudya NaveenDepartment of Veterinary Biochemistry, Faculty of Veterinary Sciences, College of Veterinary Science, Rajendranagar-500030, PVNRTVU, Hyderabad
Published in the Indian Veterinary Journal December 2020 : 97 (12) - pages 9 to 13(Received: November, 2021, Accepted: December, 2021)
Abstract
Lactoferrin (Lf) is a bioactive protein that binds with iron and is found in mammalian biological secretions. It is present primarily in the milk and is involved in various protective functions. In the present study the isolation and purification of bovine lactoferrin (BLf) was undertaken in the milk samples of Poda thurpu cow breed which has been recently recognized as native breed of Telangana state. The BLf was isolated from milk samples by using cation exchange chromatography with the help of CM- Sephadex C-50 column resin. The isolated protein was confirmed with regard to its molecular weight by using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE). The formation of single band at 80 KDa in the polymerized acrylamide gel confirmed the identity of BLf
Key Words: Poda thurpu cow milk, Lactoferrin, Cation exchange chromatography, SDS–PAGE