411/18 | Clinical & Field Article | Veterinary Microbiology
Fusion Protein of Aminoacid Mutations in Newcastle Disease Isolated from Swan Goose Caused Resistance to Infection
Naimah Putri, Rahaju Ernawati, Suwarno, Jola Rahmahani and Fedik Abdul RantamLaboratory of Virology and Immunology Department Microbiology, Faculty of Veterinary Medicine, UniversitasAirlangga, Surabaya, 60115, Indonesia
Published in the Indian Veterinary Journal October 2019 : 96 (10) - pages 53 to 55(Received: December, 2018, Accepted: June, 2019)
Abstract
Newcastle disease virus (NDV) cloacal swab were collected from unvaccinated swan goose and isolated by inoculation in Speci c Pathogen Free embryonated eggs. Haemagglutination and Haemagglutination inhibition tests were done to identify and the, samples were processed to one step Reverse Transcriptase –Polymerase Chain Reaction using primer that amplied sequences of fusion (F) gene. Deduced amino acid sequence of the cleavage site of fusion (F) protein revealed that all isolates had avirulent motifs. Amino acid mutations that occurred were analyzed to the same reference sequence. The result showed that amino acid mutations was not capable of changing the virulence of the virus
Key Words: Newcastle disease virus (NDV), amino acid, mutations and virulence