152/19 | General Article | Veterinary Microbiology
Genetic Identi cation of blactx-M Gene and blatem Gene on Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia Coli from Dogs
Luviana Kristianingtyas, Mustofa Helmi Effendi1, Wiwiek Tyasningsih and Fredy KurniawanDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Indonesia
Published in the Indian Veterinary Journal January 2020: 97 (1) - pages 17 to 21(Received: May, 2019, Accepted: September, 2019)
Abstract
The purpose of this study was to identify the ESBL producing E. coli and the characteristics of the encoding genes. 84 rectal dog swabs from several animal clinics in Surabaya, Indonesia were taken for the study. All the 84 positive E. coli samples were conrmed by the Double Disc Synergy Test (DDST) method. Molecular identi cation of blaCTX-M and blaTEM ESBL encoding genes using mutiplex Polymerase Chain Reaction (PCR). ESBL-producing E. coli bacteria from rectal swabs were found to be 9.52% (8/84). The PCR results showed that the blaCTX-M gene consisted of 8 ESBL isolates and blaTEM gene as many as 1 ESBL isolate which have the potential as a reservoir in spreading antibiotic resistance to humans and animals
Key Words: Escherichia coli, ESBL, blaCTX-M gene, blaTEM gene, Dogs