247/22 | Research Article | Veterinary Anatomy and Histology

Sea Cucumber-Based Hydroxyapatite-Chitosan Ameliorate Serum Liver Enzymes and Cytokine Levels in Albino Rats with Femoral Bone Defect

Department of Veterinary Anatomy, Faculty of Veterinary Medicine, School of Health and Life Sciences, Universitas Airlangga, Surabaya, 60115, Indonesia

Abstract

This study aimed to evaluate sea cucumber-based hydroxyapatite-chitosan (HA-Ch) as a bone graft in cases of bone defects on the variables of serum liver enzymes, IL-4, IL-6, calcium and phosphate levels. A total of 18 albino rats were used in the study, grouped into (C) control group, (T1) polyethylene glycol (PEG) group, and (T2) HA-Ch group. The treatment was carried out for 42 days, respectively. Surgical procedures and modeling of bone defects were carried out using standard methods. Data analyzed statistically using one-way ANOVA followed by post hoc Tukey multiple comparisons test (p<0.05). In result, ALP, IL-4, IL-6, calcium and phosphate levels reported significantly improved at T2 group compared to T1 and C groups. Meanwhile, ALT, AST, and GGT levels were not significant in all groups. In conclusion, HA-Ch gel can act as a bone graft to repair bone defects during the ossification process.

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