431/19 | General Article | Rats

Effect of Tadpole Serum on Thyroid Hormones and Cytotoxic T-Cell Activity in Wistar Rats: A Model of Skin Cancer

Anjani Marisa Kartikasari, Cytra Meyliana Surya Dewi, Nadiya Listyasari, Arliandra Reza Pratama, and Muhammad Thohawi Elziyad Purnama

Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia, 60115

Published in the Indian Veterinary Journal June 2020: 97 (6) - pages 34 to 37
(Received: January, 2019, Accepted: February, 2020)


A total of 20 male Wistar rats were used in this study and randomly divided into five treatment groups i.e. (C-) were injected subcutaneously with saline solution; (C+; D1; D2; D3) groups were injected subcutaneously with 20 mg/Kg BW Dimetylbenz(?)anthracene (DMBA) and tadpole serum (0; 0.27; 0.71; 1.06 ml/rats/day), respectively. Treatments were continued for a month. Serum samples were quantified with IMMULITE 2000 kit and skin organ analyzed by immunohistochemistry staining. The results showed that the dose of D2 effective for triiodothyronine (T3) and cytotoxic T-cell expression; and the dose of D3 effective for thyroxine (T4) and thyroid-stimulating hormone (TSH).

Key Words: Tadpole serum, thyroid hormones, cytotoxic T-cell, skin cancer

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