Research Article | Veterinary Clinical Medicine

Maximising Modern Diagnostic Approaches to Assess Genetic Contamination in Laboratory Rodents

Drishya Antony, Shashikant Ahire, and Arvind Ingle

Sunandan Divatia School of Science, NMIMS, Mumbai- 400056

Published in the Indian Veterinary Journal July 2022 : 99 (7) - pages 24 to 34
(Received: , Accepted: )

Abstract

An important point in the process of breeding the laboratory animals is to assess genetic purity. Eighteen different microsatellite markers were selected to test the purity of mice strains maintained at ACTREC, Navi Mumbai. Tail tissue samples were collected from ten different strains of miceand genetic integrity was monitored using simple sequence length polymorphism. Marker no. D9Mit129 showed deviation for A/J, BALB/c, C3H/J, C57BL/6 and DBA/2 mice strains whereas D4Mit53 and D8Mit4 showed deviation for A/J and C57BL/6 mice, respectively. However, since the deviation was reported from the time of importing these strains, it is considered as the true product size as other markers reveal the standard pattern for all markers. Results suggested that combination of two different microsatellites markers in a single reactions is possible to run on a single gel to differentiate the strains. It also showed that multiplex panels provide an economical and rapid way to monitor genes for genetic contamination in a mouse colony.

Key Words: Genetic monitoring,mice,Microsatellite marker,Multiplex PCR

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