148/19 | General Article | Veterinary Surgery and Radiology

Molecular Identication of Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli Isolated From Dairy Cows in East Java Province, Indonesia

Akyun Rozaqi Syah Putra, Mustofa Helmi Effendi, Setiawan Koesdarto and Wiwiek Tyasningsih

Halal Research Center, Airlangga University, Surabaya - 60115, Indonesia

Published in the Indian Veterinary Journal October 2019 : 96 (10) - pages 26 to 30
(Received: April, 2019, Accepted: June, 2019)


Occurrence of extended spectrum beta-lactamases (ESBL) in Escherichia coli (E. coli) from animal origin is a growing health concern of global signicance. The objective of this study was to determine the occurrence of ESBL producing E. coli and the characteristics of their encoding genes from 115 rectal swab samples of dairy cows from Tululungagung and Surabaya farms. All samples were positive for Escherica coli as per indole test. To con rm the ESBL the Double Disc Synergy Test (DDST). Betalactam antibiotic disk namely Amoxicylyn-clavulanate, Ceftazidime and Cefotaxime were used for DDST. Molecular identication for blaCTX-M and blaTEM ESBL encoding genes was done by used Polymerase Chain Reaction. The Escherichia coli bacteria isolated from rectal swabs of dairy cows was 5.21% (6/115). PCR results showed that blaCTX-M gene was 6 ESBL isolates and blaTEM gene was 2 ESBL isolates. It can be concluded that dairy cows can be potential as reservoir for spreading ESBL isolates to human health

Key Words: Eschericia coli, ESBL, DDST, blaCTX-M gene, blaTEM gene, PCR

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