272/22 | Research Article | Veterinary Microbiology

Isolation, Identification and Molecular Characterization of a Virulent Strain of Pasteurella multocida Isolated from a Suspected case of Hemorrhagic Septicaemia in Buffalo

Department of Veterinary Microbiology, College of Veterinary Science, Mamnoor, Warangal, P.V. NarsimhaRaoTelangana Veterinary University Telangana, India

Abstract

The present study was done to isolate, identify and molecularly characterize Pasteurella multocida from a suspected case of Hemorrhagic septicaemia (HS) in buffalo in Nellore district of Andhra Pradesh. The heart blood, piece of liver, lung, spleen and lymph nodes were collected from a 5 year old buffalo which died after showing the symptoms of high fever and respiratory distress. The samples were subjected to isolation and identification of bacterial agent through culture on suitable microbiological media and by biochemical tests. Molecular characterization of the isolate was done using Polymerase Chain Reaction. Upon cultural, biochemical and molecular characterization, the isolate was found to be Pasteurella multocida capsular type B. The isolate possessed frequently reported virulence genes viz.,ompH, oma87, fimA, ptfA, fur, tbpA, nanB and sodA. On antibiogram the isolate was found to be resistant to Co-trimoxazole, the drug of choice for HS, Tetracyclines, Penicillin, Nalidixic acid and sensitive to Cefotaxime, Ciprofloxacin, Levofloxacin. The study reports the emergence of virulent and multidrug resistant strain of Pasteurella multocida and the need for modification of the existing vaccines or vaccination strategy for prevention and control of HS.

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