59/19 | General Article | Veterinary Public Health and Epidemiology

Characterization of Cellulolytic Bacteria as Candidate Probiotic for Animal

Andreas BernyYulianto, WidyaParamitaLokapirnasari, RifkyNajwan, Khoirul Huda, Hana CipkaPramudaWardhani, and Nabil FarizNoor Rahman

Department of Animal Husbandry, Faculty of Veterinary Medicine, UniversitasAirlangga, Mulyorejo Street, Surabaya 60115, Indonesia

Published in the Indian Veterinary Journal August 2019 : 96 (8) - pages 29 to 31
(Received: February, 2019, Accepted: April, 2019)


This study was aimed to identify cellulolytic isolates from local bovine rumen. Cellulolytic activities of that isolate were evaluated to determine the activities of endoglucanase, exoglucanase and β-glucosidase. Genotypic testing was conducted by analysing 16S rDNA and biochemical identication of VITEK 2 microbial identification system version: 05.01 (BioMérieux). DNA of sample bacteria was isolated and further amplied in vitro with the PCR method. The nucleotide sequence of 16S rDNA fragment was determined through the sequencing method. The result was then compared with GenBank database to recognize the type of the sample bacteria. Based on the result, the cellulolytic isolate was identied as Enterobacter cloacae.

Key Words: cellulolytic enzyme activity; 16S rDNA; biochemical test

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