342/19 | General Article | Animal Biotechnology
Molecular Characterization of 18S SSU rRNA Cryptosporidium spp. Infected Dogs in Surabaya City - Indonesia
Romy Muhammad Dary Mufa, Nunuk Dyah Retno Lastuti, Fedik Abdul Rantam, Lucia Lucia Tri Suwanti, Endang Suprihati, Didik Handijatno, and Mufasirin MufasirinFaculty of Veterinary Medicine, Airlangga University, Kampus C Unair, Mulyorejo, Surabaya 60115, East Java, Indonesia
Published in the Indian Veterinary Journal May 2020: 97 (5) - pages 24 to 27(Received: September, 2019, Accepted: January, 2020)
Abstract
This study aims to characterize the 18S SSU rRNA Cryptosporidium spp. in dogs in the Surabaya City - Indonesia. A total of 70 dog faecal samples were taken from animal hospitals and animal clinics. Cryptosporidium spp. isolated and identified microscopically then confirmed by Polymerase Chain Reaction and sequencing. A total of 40 samples were found to have Cryptosporidium spp. oocysts, measuring 2-6 ?m then confirmed by PCR. Six positive samples were sequenced and analyzed by blasting. The results showed similarity with Cryptosporidium canis and has a kinship with Cryptosporidium canis in Japan, China, Brazil, Australia, England, Romania, Mexico, France, Thailand, the Philippines and USA.
Key Words: Cryptosporidium, dog, molecular characterization.